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Search for "fluorescent tag" in Full Text gives 8 result(s) in Beilstein Journal of Organic Chemistry.
Naphthalene diimide–amino acid conjugates as novel fluorimetric and CD probes for differentiation between ds-DNA and ds-RNA
Beilstein J. Org. Chem. 2020, 16, 2032–2045, doi:10.3762/bjoc.16.170
- significant Stokes shifts of emission (+60 nm) characterised by good quantum yield in aqueous solution. Thus, NDIs 3a and 3b are novel intensively fluorescent non-natural amino acid probe molecules with both, N- and C-termini available for incorporation into any peptidoid construct requiring a fluorescent tag
Nonenzymatic synthesis of anomerically pure, mannosyl-based molecular probes for scramblase identification studies
Beilstein J. Org. Chem. 2020, 16, 1732–1739, doi:10.3762/bjoc.16.145
- , whereas the probe MPC-2 bears a fluorescent tag. Chemical structures of commercially available (S)-citronellol (Cit), 4,4′-dihydroxybenzophenone (BZP), ᴅ-mannose (Man), and 1,12-dodecanediol (Dod), the main starting materials in the synthesis of MPC-1 and MPC-2. The synthetic route leading to compounds
Novel solid-phase strategy for the synthesis of ligand-targeted fluorescent-labelled chelating peptide conjugates as a theranostic tool for cancer
Beilstein J. Org. Chem. 2018, 14, 2665–2679, doi:10.3762/bjoc.14.244
- ligand for biomarkers, a fluorescent tag and a chelating moiety for tethering cargo in a continuous process using solid-phase peptide synthesis is poorly developed. Traditional solid-phase peptide synthesis methods for preparation of bioconstructs employ orthogonally protected functional moieties present
- ) amino protecting group before attachment of fluorescent tag with the peptidic spacer. This results in premature cleavage of the peptide chain and loss of chemical yield during the bioconjugate synthesis. Further, attaching a radiotracer chelating core containing acid sensitive functional groups and the
- amino acid cysteine is also cumbersome and challenging. Recently, Low et al. reported synthesis of various targeted conjugates in which fluorescent tag [37] has been attached in a solution phase reaction. Also, they have reported the synthesis of ligand-conjugated peptides containing a radiotracer
Creating molecular macrocycles for anion recognition
Beilstein J. Org. Chem. 2016, 12, 611–627, doi:10.3762/bjoc.12.60
- emerge but when they do, they represent plenty of scope for creative chemical design. At the time, I observed that the triazole linkages were used to bring together two modules, be they a fluorescent tag “clicked” onto a protein or redox-active group “clicked” together with a polymer. But I also thought
Synthetic strategies for the fluorescent labeling of epichlorohydrin-branched cyclodextrin polymers
Beilstein J. Org. Chem. 2014, 10, 3007–3018, doi:10.3762/bjoc.10.319
- prepared either in well water-soluble form or as gels of high swelling. Two versatile synthetic strategies for introducing a fluorescent tag (rhodamine, fluorescein, nitrobenzofuran or coumarin) into the water-soluble epichlorohydrin branched cyclodextrin polymers were worked out and compared. The
- introduction of methyl groups into the CD polymers generates structures with peculiar properties, such as enhanced solubility and solubilizing effect, although only a few examples are found in the literature [14][15]. The introduction of a fluorescent tag into a water-soluble epichlorohydrin-branched CD
A small azide-modified thiazole-based reporter molecule for fluorescence and mass spectrometric detection
Beilstein J. Org. Chem. 2014, 10, 2470–2479, doi:10.3762/bjoc.10.258
- receive BNS (6). Furthermore, we utilized N-(3-azidopropyl)-7-nitrobenzo[c][1,2,5]oxadiazol-4-amine (NBD, 9), a cheap fluorophore previously used for probes [1][31] or as fluorescent tag [32][33] (Figure 2). UV–vis spectra of all substances were recorded in an aqueous solution containing 20% THF (v/v
Design and synthesis of tag-free photoprobes for the identification of the molecular target for CCG-1423, a novel inhibitor of the Rho/MKL1/SRF signaling pathway
Beilstein J. Org. Chem. 2013, 9, 966–973, doi:10.3762/bjoc.9.111
- and fibrosis. The molecular target for these compounds is unknown. To facilitate its identification, a series of tag-free photoaffinity probes was designed and synthesized, each one containing a photoactivatable group and an acetylenic end group for subsequent attachment to a fluorescent tag using
- current hypothesis regarding the mechanism of action of 1 (redistribution of MKL primarily into the cytosol), which may require an intact actin cytoskeleton or nucleus. We thus elected to design photoaffinity probes that were tag-free, i.e., lacking either a biotin or fluorescent tag [17]. There are a
- . Following exposure to UV light, the cells would be lysed, releasing the labeled proteins bound covalently to the probe (B). Click chemistry would then be applied to covalently attach a biotin or fluorescent tag for visualization and/or isolation (C). Any isolated proteins would be digested and identified by
Peptoids and polyamines going sweet: Modular synthesis of glycosylated peptoids and polyamines using click chemistry
Beilstein J. Org. Chem. 2013, 9, 56–63, doi:10.3762/bjoc.9.7
- coupling sequence resulted in the peptoids, which were further modified with rhodamine B (Rho-CO2H) as an easily accessible and versatile fluorescent tag. Rhodamine B was coupled to the N-terminus in order to provide a label for future biological applications, such as the study of the cellular uptake. For
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